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Used Prime for many years but decided to try Ultimate 2 - 3 months ago based on Cory's recommendation. I have not noticed any changes in the fish behavior and while not as cost effective as Prime I will continue with Ultimate for the added benefits (buffering, slime coat) and not having to deal with Prime's smell that was left on my fingers after use.
It works great! One thing to note however is that it does not remove ammonium hydroxide (NH+). This has made it annoying for testing my aquarium water having false alarms.
I bought this because Cory speaks so highly of it. Nitrite has spiked for reasons I am so far unsure of in my established tank. I've been using Ultimate as a dechlorinator for the added benefit of it detoxifying nitrite while my biological filtration gets better. So far so good!
Easy, simple to use. Takes care of many things. Love Ultimate and I use it instead of Prime.