When should I turn on and off the CO2 in my aquarium?
Plants use CO2 when there is light to photosynthesize. However, the process reverses at night and becomes the respiration cycle, in which plants consume oxygen and sugars and release CO2. Therefore, we want to shut off the CO2 regulator when the aquarium light is off. For more optimized CO2 usage, program the regulator’s timer to turn on 1–2 hours before the light comes on and turn off 1 hour before the light shuts off. (If you only have one timer, you can use the same timer with a power strip so that the light and regulator turn on and off at the same time.)
For more information on CO2 systems in high tech planted tanks, read the full article.